Isolation of DNA from saliva of betel quid chewers using Treated Cards
Salvador, J.M. and De Ungria, M.C.A.
DNA Analysis Laboratory, Natural Sciences Research Institute, University of the Philippines, Quezon City
Journal of Forensic Science (in press). Betel quid (BQ) chewing, a common tradition in tropical areas, often poses a problem during collection and DNA analysis of buccal samples from many indigenous communities for population genetic studies and in forensic analysis of chewed BQ residues. This study evaluated the use of FTA™ card, a chemically treated filter paper, in collecting buccal samples from long-term BQ users and subsequent PCR-based analysis using nine STR markers. A low overall success rate of amplification was observed in the samples extracted
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